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HHMI: THE FIRST 3D technology that allows the study of living cells ... A MAJOR STEP IN THE ZINC-METHANE

Scientific Howard Hughes Medical Institute (HHMI) in the United States managed to obtain 3D images of living cells in action, a technique that could help study these biological units like never before, " For the first time we have a technology that allows us to look at three dimensions the complexity of the case, the rate at which happens within cells, "said Eric Betzig, HHMI physicist who led the research. The study was published in the journal Nature Methods.

This technique is based on flat or cross lighting by photon excitation microscopy, where emissions of light called Bessel beams, when projected to the cells from the lateral to the plane of focus of the team, obteniene multiple layers or levels of cell 2D which when joined together create a 3D image. cytology Other techniques such as wide-field microscopy and confocal offer high resolutions, but have disadvantages in spatial resolution and lighting samples.

Such constraints are the illumination of fixed cells from the bottom or the possibility of eliminating cells with overexposure of light, " When you try to study living cells for some period of time, the light itself begins to damage cells and eventually curl up and die. You can get much information by looking at fixed and dead cells, but there is much we can learn to see things moving , "said Eric Betzig. For now, this technology represents a major step for the study of living cells.





SOURCE:
http://www.bbc.co.uk/mundo/noticias/2011/03 / 110307_ciencia_celulas_3d_sao.shtml
http://sanacion-estrellaanamaria.blogspot.com/2011/03/nuevo-microscopio-capta-video-3d-de-una.html